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KMID : 0364819910290010034
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Korean Journal of Microbiology
1991 Volume.29 No. 1 p.34 ~ p.39
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cis-Diamminedichloroplatinum (II) (CDDP) Inhibits Bluetongue Virus (BTV) Core Associated Transciptase Activity
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Yang Jai-Myung
JaRue S. Manning
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Abstract
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1
The BTV core associated transcriptase activity, assayed by acid precipitable counts, was reduced to an undetectable level after treat the core with 100 uM CDDP. When the RNA transcripts prepared from the CDDP treated BTV core were analysed on agaroseurea gel, it was observed that the band intensity of the large size RNA was reduced while the band intensity of the small size RNA was enhanced. Northern blot analysis showed that much of the small size RNAs appeared to be prematurely terminated transcripts. These results suggest that CDDP adduction to the template RNA blocks chain elongation process of the virion bound transcriptase that is ultimately responsible for the inactivation of BTV infectivity.
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